Abstract
A new coumarin-based amine, 4-aminomethyl-6, 7-dimethoxycoumarin, was synthesized as a precolumn fluorescence derivatization reagent for carboxylic acids. The derivatization using the amine in a two-phase medium of water and ethyl acetate was examined. The two-phase derivatization method with the amine was found to be of value since it allows simple and rapid HPLC assays of carboxylic acids with a reasonable sensitivity and reparability. The detection limit was determined to be 20-50fmol per 10μl injection volume at a signal-to-noise ratio of 3 under partially optimized conditions. The method was also applied to the assay of fatty acids in human serum.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.