Abstract
Introduction.Bacteriochlorins are the most promising photosensitizers absorbing in the near-infrared spectral region. Their use can enhance the efficiency of photodynamic therapy due to the deeper penetration of radiation into the tumor.Objectiveto conduct a preclinical study of the photoinduced antitumor activity and biodistribution of Bacteriosens.Materials and methods.Bacteriosens is a preparation based on meso-tetra(3-pyridyl)bacteriochlorin absorbing at 747 nm. Photoinduced cytotoxicity was investigated in vitro using human tumor cells: A549, Hep 2, BT-474, MCF-7, SK-BR-3, PC3, and EJ and murine tumor cells: S37, C26, and LLC. In vivo studies were performed in mice with large and small tumors (S37, LLC, and C26).Results.In vitro investigation show that bacteriosens during optical irradiation led to the effective suppression of tumor cell growth in culture (the IC50 value varied from 0,08μМ to 1,21 μМ) and had no toxicity without exposure to light. The effective photodynamic therapy regimen using Bacteriosens in mice with inoculated small and large tumors of different genesis resulted in regression of a primary tumor node on 90–100 % of the animals in the absence of tumor recurrence within 90 days after treatment.Conclusion.Bacteriosens is a promising agent for the photodynamic therapy of small and large tumors; it can be successfully used as an alternative, organ-sparing minimally invasive treatment for malignant tumors, including prostate cancer.
Highlights
Objective to conduct a preclinical study of the photoinduced antitumor activity and biodistribution of Bacteriosens
In vitro investigation show that bacteriosens during optical irradiation led to the effective suppression of tumor cell growth in culture and had no toxicity without exposure to light
РОССИЙСКИЙ БИОТЕРАПЕВТИЧЕСКИЙ ЖУРНАЛ Russian journal of biotherapy therapy regimen using Bacteriosens in mice with inoculated small and large tumors of different genesis resulted in regression of a primary tumor node on 90–100 % of the animals in the absence of tumor recurrence within 90 days after treatment
Summary
ДЛЯ ФОТОДИНАМИЧЕСКОЙ ТЕРАПИИ ЗЛОКАЧЕСТВЕННЫХ НОВООБРАЗОВАНИЙ, В ТОМ ЧИСЛЕ РАКА ПРЕДСТАТЕЛЬНОЙ ЖЕЛЕЗЫ. «Бактериосенс» – препарат на основе мезо-тетра(3-пиридил)бактериохлорина, поглощающий при 747 нм. Исследования in vivo проводили на мышах с опухолями S37, LLC, C26, большого и малого размера. В системе in vitro «Бактериосенс» при оптическом облучении приводил к эффективному подавлению роста опухолевых клеток в культуре (величина ИК50 варьировала от 0,08 мкМ до 1,21 мкМ) и характеризовался отсутствием токсичности без воздействия света. Фотодинамическая терапия с «Бактериосенсом» у мышей с инокулированными опухолями (малого и большого размера) различного генеза, проведенная в эффективном режиме, приводила к регрессии первичного опухолевого узла на 90–100 % при отсутствии рецидива опухоли в течение 90 сут после лечения. «Бактериосенс» является перспективным препаратом для фотодинамической терапии опухолей малого и большого размера, может успешно использоваться в качестве альтернативного, органосохраняющего малоинвазивного метода лечения злокачественных новообразований, в том числе рака предстательной железы.
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