Pre-clinical test for non-invasive (in vitro) blood glucose levels measuring at visible light wavelengths

Abstract

The wavelength convention for non-invasive blood glucose measurement remains inconclusive. We have done this experimental observation to measure wavelength candidate performance for non-invasive blood glucose level measurement optical device in the visible range. We run this observation in Electronic Material Physics Lab, IPB University, Bogor, July 2019. We have measured two levels the blood glucose level control in cuvettes using LEDs and photodiodes from Thorlabs. The LEDs are between 450 nm to 670 nm. BioRad has provided The 100 mg/dl and 3030 mg/dl blood glucose level control for this study. We have modulated The LEDs intensity in 10 different values in a ladder setting. We have used a custom made probe to make sure there is no residual light effect from the environment. We have analysed the photodiodes readings using multi formulated regression to find the appropriate equation for predicting blood glucose level. We have found that LED in 645 nm wavelength gave the highest accuracy at 0.71. The second highest accuracy is 0.70 by LED 610 nm. The R^2 value is 0.97. We concluded that LED 645 nm might appropriate for non-invasive blood glucose level measurement optical device. Further research shall confirm this trial with human observation.