Preclinical Hemotoxicological Profile Investigations of an Ayurvedic Medication Following Long-Term Adminidration to Male Sprague-Dawley Rats

Abstract

Kutajarista (KTJ) is an Ayurvedic preparation used as a traditional medicine in ulcerative colitis in the rural population. A self-made alcoholic preparation derived from the Indian Medical System known as Kutajarishta. The main ingredient in this polyherbal combination is Holarrhena antidysenterica Wall. Ex DC (Family: Apocynaceae). It is used to treat gastrointestinal disorders, diarrhea, and amebic dysentery. Ayurvedic practitioners have been using Kutaja formulations for centuries, as they have been found to be quite helpful clinically in certain diseases. This study was conducted using mainly old literature as a basis method. At a dose of 40 milliliters per kilogram, KTJ was given chronically to male Sprague-Dawley rats in order to determine its toxicological characteristics. Following a continuous 28-day KTJ preparation administration, the following toxicological alterations were observed. Several erythrocytic parameters were found in this experiment. The anemia panel studies' findings are as follows: The total amount of red blood cells in the male rat has decreased by [7.98%]. This drop was noticeable (p=0.083) even if it was not statistically significant. There is a [8.08 %] decrease in the Hemoglobin content of the blood of the male rat, the decrease though not significant yet it was prominent (p=0.097). There is a [8.03 %] decrease in the Hematocrit level of the blood of the male rat, the decrease though not significant yet it was prominent (p=0.072). There is a statistically insignificant (p=0.882) [0.19 %] increase in the Mean corpuscular volume, a red cell index of the male rat. There is a statistically insignificant (p=0.420) [3.02 %] decrease in the Mean corpuscular hemoglobin, a red cell index of the male rat. A statistically insignificant (p=0.203) [8.50 %] increase in the red cell volume distribution width, a red cell index of the male rat, is observed, along with a negligible [3.01 %] decrease in the Mean corpuscular hemoglobin concentration, a red cell index of the male rat, which was statistically not at all significant (p=0.561).