Abstract
The proprietary DA-5512 formulation comprises six herbal extracts from traditional oriental plants historically associated with therapeutic and other applications related to hair. Here, we investigated the effects of DA-5512 on the proliferation of human dermal papilla cells (hDPCs) in vitro and on hair growth in C57BL/6 mice and conducted a clinical study to evaluate the efficacy and safety of DA-5512. DA-5512 significantly enhanced the viability of hDPCs in a dose-dependent manner (p < 0.05), and 100 ppm of DA-5512 and 1 μM minoxidil (MXD) significantly increased the number of Ki-67-positive cells, compared with the control group (p < 0.05). MXD (3%) and DA-5512 (1%, 5%) significantly stimulated hair growth and increased the number and length of hair follicles (HFs) versus the controls (each p < 0.05). The groups treated with DA-5512 exhibited hair growth comparable to that induced by MXD. In clinical study, we detected a statistically significant increase in the efficacy of DA-5512 after 16 weeks compared with the groups treated with placebo or 3% MXD (p < 0.05). In conclusion, DA-5512 might promote hair growth and enhance hair health and can therefore be considered an effective option for treating hair loss.
Highlights
Alopecia or hair loss is a dermatologic disease, which affects men and women, and often affects self-esteem and personal attractiveness, potentially leading to depression and other negative effects [1]
The present study was undertaken to investigate the pharmacological effects of DA-5512 on the proliferation of human dermal papilla cells in vitro and its effects on C57BL/6 mice and to evaluate its efficacy and safety when administered to human subjects
DA-5512 and MXD (1 μM) significantly enhanced the viability of human dermal papilla cells (hDPCs) compared with the control group (Figure 1)
Summary
Alopecia or hair loss is a dermatologic disease, which affects men and women, and often affects self-esteem and personal attractiveness, potentially leading to depression and other negative effects [1]. Tribulus terrestris Pinus densiflora Emblica officinalis Pueraria thunbergiana Zingiber officinale. Several herbal formulations are commercially available to treat alopecia, such as hair tonics, hair-growth stimulants, hair conditioners, hair-cleansing agents, and antidandruff agents [14]. The proprietary DA-5512 formulation includes herbal extracts from Thea sinensis L., Emblica officinalis, Pinus densiflora, Pueraria thunbergiana, Tribulus terrestris, and Zingiber officinale. Phyllanthus emblica L. is a potent inhibitor of 5α-reductase, which promotes the growth of the hair of C57BL/6 mice [19]. The roots and flowers of P. thunbergiana are used in traditional medicine because of their medicinal properties [21, 22], and a study of a murine model of hair loss demonstrates their antiandrogenic activity and proliferative effects on hair growth [19]. The present study was undertaken to investigate the pharmacological effects of DA-5512 on the proliferation of human dermal papilla cells (hDPCs) in vitro and its effects on C57BL/6 mice and to evaluate its efficacy and safety when administered to human subjects
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