Abstract
In discussing the theories and mechanism of sectioning, it is pointed out that virtually no concrete knowledge exists on the subject. The function of different mechanisms and their role in cutting different types of tissue, and the value of microtome knives versus safety razor blades is discussed. Razor blades failed to cut precise sections; a technic for sharpening a knife to give precise sections is outlined, pointing out errors to avoid in sharpening and sectioning. Various types of knife edges are illustrated by photomicrographs. The procedure of sharpening and sectioning technics for critical results is explained. The mechanism of cutting in wood appeared to be of the crushing and tearing type, indicating the necessity of final polishing in sharpening.
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