Abstract
Precipitation with cetyltrimethylammonium bromide followed by precipitation with ethanol:salt provides a quick, simple, and effective method for preparing labelled nucleic acid products of DNA polymerase reactions for analysis by Cs 2SO 4 equilibrium density gradient centrifugation. Results obtained using this treatment show that this approach is superior to other methods of preparing products of endogenous DNA polymerase reactions from relatively crude particulate fractions such as disrupted virions.
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More From: Biochemical and Biophysical Research Communications
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