Abstract
Inulin and fructooligosaccharides (FOS) are fructans a reserve polysaccharides that have a great economic interest in the food and pharmaceutical industries. Cichorium endivia, also known as “escarole”, belongs to the family Asteraceae and normally is used as a salad, forage crops and raw material for the production of fructose. The objective of this work was to extract, characterize chemically, and evaluate the prebiotic activity in vitro from C. endivia roots FOS. By colorimetric assays, we to determined its chemical composition with 85.4% of total sugars containing 72.2% of fructose. HSQC-Q NMR was used to determine the monosaccharide composition. The NMR and off-line ESI-MS analysis showed that C. endivia FOS has a degree of polymerization equal to 6 and 7, respectively. In vitro prebiotic test showed that the same was fermented by all probiotic bacteria evaluated and the results showed that C. endivia FOS is a promising source of prebiotic fructans.
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