Pre-treatment with 1-methylcyclopropene alleviates methyl bromide-induced internal breakdown, softening and wall degradation in blueberry

Abstract

Methyl bromide (MeBr) fumigation is the most common quarantine treatment used to control fruit flies in blueberry. Recent studies suggest that the treatments may increase decay and softening during transport, distribution and retail. We evaluated whether the ethylene action inhibitor 1-methylcyclopropene (1-MCP) could counteract the detrimental effects caused by MeBr. ‘Jewel’ and ‘Emerald’ blueberries with 100% surface blue color were harvested and treated with 1-MCP (1 μL L−1 12 h, 4 °C), MeBr (32 g m−3, 3 h, 21 °C), or 1-MCP followed by MeBr. Untreated berries were used as a control. Fruit was stored for 0, 7 or 14 d at 2 °C and internal breakdown, firmness, respiration, weight loss, color, soluble solids, acidity and the total ascorbic acid (AsA), anthocyanin and glutathione (GSH) concentrations were determined. We also assessed pectin solubility by sequential cell wall extraction and neutral sugar composition. MeBr exposure exacerbated internal breakdown and respiration after long-term storage. These effects were significantly reduced by pre-treatment with 1-MCP, indicating that MeBr-induced damage requires ethylene action. 1-MCP application prior to MeBr fumigation also prevented berry softening by delaying solubilization of cell wall uronic acids and galactose. The combination of 1-MCP followed by MeBr caused no detrimental effects on fruit surface color, anthocyanin, weight loss, soluble solids or acidity. MeBr fumigation reduced total GSH concentrations regardless of 1-MCP, indicating that the improved quality retention could not be attributed to the detoxification of the xenobiotic by this compound and was more likely due to inhibition of ethylene-dependent over-ripening and senescence symptoms. Pre-treatment with 1-MCP may be useful to alleviate MeBr-induced deterioration in blueberry.