Pre-parturition profile of steroids and prostaglandin in cows with or without foetal membrane retention

Abstract

Retained foetal membranes in cattle is one of the most common complications associated to the reduction in milk yield and impaired fertility in dairy cattle. In order to determine some endocrine mechanisms controlling parturition and delivery of foetal membranes, plasma concentrations of steroids and prostanoids were determined in 20 healthy Holstein cows. Samples were taken within the interval of 5 days pre-parturition to 12 h after calving. Progesterone (P 4) levels were similar in cows with (PR) and without (NPR) placental retention. While the estradiol-17β (E 2) peak at parturition was lower in PR than in NPR cows, cortisol levels were greater in PR cows 12 and 24 h pre-parturition. The Prostaglandin F 2α metabolite (PGFM) levels were higher at parturition in NPR compared with the PR group, but 12 h later, these levels in the PR group increased so that concentrations were greater as compared with NPR cows. The Prostaglandin E 2 metabolite (PGEM), 24, 48 and 72 h pre-parturition, were higher in PR cows. However, the PGFM:PGEM ratio was greater in cows up NPR at all time when included, indicating the importance of higher levels of Prostaglandin F 2α (PGF 2α) than Prostaglandin E 2 (PGE 2) for normal placental delivery. In conclusion, placental retention was related to both estrogen and PGF 2α deficiency, which may be a consequence of metabolic stress leading to PGE 2 and maternal cortisol synthesis before parturition.