Pre-harvest sprouting resistance and haplotype variation of ThVp-1 gene in the collection of wheat-wheatgrass hybrids.

A. A. Kocheshkova,A. A. Pochtovyy,G. I. Karlov,V. I. Belov,P. Yu. Kroupin,V. P. Upelniek,M. G. Divashuk,M. S. Bazhenov,Jaswinder Singh

doi:10.1371/journal.pone.0188049

A. A. Kocheshkova, A. A. Pochtovyy + Show 7 more

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https://doi.org/10.1371/journal.pone.0188049

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Abstract

The germplasm collection of 87 wheat-wheatgrass hybrids developed in Tsitisin Main Botanical Garden (Russia, Moscow) was evaluated for resistance to pre-harvest sprouting (PHS) by spike sprouting (SS) and germination index (GI) assays as well as for spike and grain features. The PHS resistance variation and haplotype polymorphism of the wheatgrass ThVp-1 and wheat TaVp-1B genes orthologues of Vp-1 was revealed in the studied collection. Four haplotypes of ThVp-1 were revealed: ThVp-1a (41% of the entries), ThVp-1b (13%), ThVp-1c (29%), and ThVp-1d (15%). The association between the allelic state of ThVp-1 and PHS resistance in the wheat-wheatgrass hybrids was shown: haplotype ThVp-1d of the wheatgrass Vp-1 gene is significantly associated with reduced PHS in the wheat-wheatgrass hybrids (mean SS 0.33, mean GI 0.64). The resistant entries may be perspective as a source of PHS resistance in the development of commercial cultivars of perennial wheat.

Highlights

Wheat-wheatgrass hybrids are partial amphidiploids produced by crossing Trtitcum species and various wheatgrass species
pre-harvest sprouting (PHS) resistance is determined by two groups of traits: spike morphology and grain characteristics [15, 16, 62, 67,68,69,70,71]
Most entries showed susceptibility that may pose a serious problem in wheat-wheatgrass hybrid either as a source of genetic variability in wheat breeding or as a crop in regions with conditions favorable to PHS

Summary

Introduction

Wheat-wheatgrass hybrids are partial amphidiploids produced by crossing Trtitcum species and various wheatgrass species. Bread wheat (T. aestivum L., 2n = 6x = 42, genome composition BBAADD) and intermediate wheatgrass (Thinopyrum intermedium (Host) Barkworth & D.R. Dewey, 2n = 6x = 42, genome composition JvsJvsJrJrStSt) or tall wheatgrass (Th. ponticum (Podp.) Z.-W. 2n = 10x = 70, genome composition JJJJJJJsJsJsJs) are the most commonly used for the development of wheat-wheatgrass hybrids. The genetically stable wheat-wheatgrass hybrids are mainly octaploid (2n = 56), carrying 42 chromosomes of wheat and 14 chromosomes from various subgenomes of wheatgrass. Different wheat-wheatgrass amphidiploids carry various combinations of wheatgrass chromosomes [1,2,3,4,5]

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