Pre- and postpartum supplemental DL-α-tocopheryl acetate effects on placental and mammary vitamin E transfer in sheep

Abstract

Twenty-six pregnant ewe lambs were used to study the effect of four treatment (T 1 to 4) levels of DL-alpha-tocopheryl acetate (0, 15, 30, and 60 IU.lamb-1.d-1) supplemented 28 d prepartum through 28 d postpartum on placental and mammary gland transfer of alpha-tocopherol. Blood samples were first obtained on d 28 prepartum and thereafter with milk samples at designated intervals up to 28 d postpartum for alpha-tocopherol analyses. Similarly, blood samples were obtained from neonatal lambs on d 1 before suckling and on d 3, 14, and 28 postpartum. Ewe lamb serum tocopherol concentrations averaged .66 micrograms/mL before T administration; 28 d later averages were .93, 1.94, 2.63, and 4.07 micrograms/mL (linear, P < .05) for T 1 to 4, respectively. Supplemental vitamin E had no effect (P > .05) on serum alpha-tocopherol of neonatal lambs, indicating inefficient placental transfer. By d 3, lamb serum tocopherol increased to 1.41, 1.84, 2.43, and 4.46 micrograms/mL (linear, P < .05) for T 1 to 4 following consumption of colostrum and declined to .46, .74, 1.10, and 1.68 micrograms/mL (linear, P < .05) by d 28. On d 1 there was a linear (P < .05) treatment effect on tocopherol in colostrum, with a low concentration of 3.30 micrograms/mL for the control (T 1) and a high of 9.55 micrograms/mL for T 4.(ABSTRACT TRUNCATED AT 250 WORDS)