PRDM14, a Zinc Finger Protein, Regulates Cancer Stemness.

Abstract

PRDI-BF1 and RIZ homology (PR) domain zinc finger protein 14 (PRDM14) contains a PR domain related to the SET methyltransferase domain and zinc finger motifs. PRDM14 maintains stemness in embryonic stem cells and primordial germ cells via epigenetic mechanisms. PRDM14, however, is not expressed in normal differentiated tissues. We and other groups previously reported that PRDM14 expression is markedly higher in some types of cancers compared to the corresponding normal tissues. PRDM14 confers stem cell-like characteristics upon cancer cells, such as sphere formation, dye efflux, chemotherapy resistance, proliferation, and distant metastasis. Cancer stem cells (CSCs) are thought to be responsible for tumor initiation, drug and radiation resistance, invasive growth, metastasis, and tumor relapse, which are the primary causes of cancer-related deaths. Because CSCs are also thought to be resistant to conventional therapies, an effective and novel therapeutic approach for CSCs is imperative.RNAi silencing of PRDM14 expressed by breast and pancreatic cancer cells reduced tumor size and distant metastasis of these cells in nude mice. Inhibition of PRDM14 expression by cancer cells may be an effective and radical therapy for solid cancers. In this chapter, we discuss methods for studying CSC-like properties in cancer cells and describe the use of siRNA with a drug delivery system by systemic injection in vivo.