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Abstract
The present study examined the role of the PRDI-BF1-RIZ (PR) domain of tumor suppressor retinoblastoma protein-interacting zinc finger gene 1 (RIZ1) as an anticancer domain and its ability to induce apoptosis in esophageal squamous cell carcinoma (ESCC) cells. The TE13 ESCC cell line was transfected with pcDNA3.1(+) eukaryotic expression vectors bearing the open reading frames of either the human RIZ1 gene or the PR domain, and the mRNA and protein expression levels were then detected using quantitative reverse transcription polymerase chain reaction and western blotting, respectively. The rate of apoptosis was determined by flow cytometry and the cell invasion ability was determined by an invasion assay. RIZ1 and the PR domain induced apoptosis and reduced the cell invasion ability (P<0.01). These findings indicate that the RIZ1 gene possesses anticancer activity in the PR domain, which may be important in inhibiting the development of ESCC.
Highlights
The retinoblastoma protein‐interacting zinc finger (RIZ) gene was identified by Bird [1] following the application of retinoblastoma (Rb) probes in combination with Rb protein to screen for separation‐of‐function mutants
Human retinoblastoma protein‐interacting zinc finger gene 1 (RIZ1) and positive regulatory (PR) domain eukaryotic expression vectors were constructed to investigate whether the PR domain of the tumor suppressor RIZ1 has the ability to induce apoptosis and reduce cell invasion ability in esophageal carcinoma cells
The RIZ1 and PR domain mRNA and protein expression levels in TE13 cells transfected with pcDNA3.1(+)/RIZ1 or pcDNA3.1(+)/PR were significantly higher compared with untransfected and empty vector‐transfected cells (P
Summary
The retinoblastoma protein‐interacting zinc finger (RIZ) gene was identified by Bird [1] following the application of retinoblastoma (Rb) probes in combination with Rb protein to screen for separation‐of‐function mutants. Fluorescence in situ hybridization located the gene to human chromosome 1p36. Due to the presence of various transcription initiation sites, the RIZ gene encodes two proteins, RIZ1 and RIZ2. RIZ1 contains a positive regulatory (PR) domain, whereas RIZ2 Human RIZ1 and PR domain eukaryotic expression vectors were constructed to investigate whether the PR domain of the tumor suppressor RIZ1 has the ability to induce apoptosis and reduce cell invasion ability in esophageal carcinoma cells
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