Pragia

Abstract

Pra' gi.a . L. fem. n. Pragia of Prague, the city in which strains of the genus were identified. Proteobacteria / Gammaproteobacteria / Enterobacteriales / Enterobacteriaceae / Pragia Gram‐negative, oxidase‐negative, catalase‐positive, peritrichously flagellated rods conforming to the definition of the family Enterobacteriaceae . Facultatively anaerobic. Colonies approximately 0.5 mm in diameter are formed on nutrient, Endo, and MacConkey agars. Colonies formed on desoxycholate‐citrate agar have a black center. Nonhemolytic on sheep blood agar. A Shigella ‐like odor is produced on nutrient agar. A pigsty‐like odor is produced on Endo agar overlaid with a sloping surface of agar. Growth at 4°C, but not at 42°C . Optimal growth is obtained at temperatures between 22 and 37°C. Fermentative, forms acid without gas from D ‐glucose and D ‐galactose. Motile, reduces nitrates to nitrite, utilizes citrate, produces hydrogen sulfide, and oxidizes gluconate . The majority of strains is positive for the methyl red test and delayed positive for acid production from glycerol and myo ‐inositol. Negative reactions in tests for indole production, Voges–Proskauer test, phenylalanine deaminase, lysine and ornithine decarboxylases, arginine dihydrolase, urease, deoxyribonuclease, malonate, ONPG, growth in KCN, and acid production from L ‐arabinose, cellobiose, dulcitol, lactose, maltose, D ‐mannitol, D ‐mannose, melibiose, raffinose, L ‐rhamnose, D ‐sorbitol, D ‐sorbose, starch, sucrose, and trehalose. Additional biochemical reactions are given in Table 1 of the chapter on Enterobacteriaceae . The mol % G + C of the DNA is : 46–47. Type species : Pragia fontium Aldová, Hausner, Brenner Kocmoud, Schindler, Potužníková, and Petráš 1988a, 187.