Abstract
Brucella species are short oval rods (coccobacilli) and are associated with infected animals of farmers, veterinarians, slaughterhouse workers, inadequately heated meat, raw milk, and cream and cheese made from nonpasteurized milk. Brucella abortus cannot grow in media containing thionil, but others can grow. The Brucella milk ring test can be used safely and identify milk samples that contain antibodies to the bacterium originating from an infected animal. A biphasic system with both agar and liquid in the bottle can be used in the selective enrichment isolation of Brucella. Morphological analysis can be performed from enriched broth and Brucella species are isolated using selective/differential medium Brucella agar. Brucella are identified from isolates using phenotypic and foodomics techniques. Brucella species are highly infective bacteria and there is a high risk of infections for laboratory staff working with Brucella. The isolation of Brucella should be restricted to official specialist public health and clinical laboratories.
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