Practical Protein Crystallography

Abstract

Laboratory Techniques: Preparing Protein Samples. Protein Crystal Growth. Crystal Storage and Handling. Crystal Soaking. Anaerobic Crystals. Data Collection Techniques: Preparing Crystals for Data Collection. Optical Alignment. X-Ray Sources. Preliminary Characterization. Heavy Atom Derivative Scanning with Film. Overall Data Collection Strategy. Overview of Older Film Techniques. Four-Circle Diffractometer Data Collection. Area Detector Data Collection. Image Plate Data Collection. Synchrotron Radiation Light Sources. Data Reduction. Computational Techniques. Terminology. Basic Computer Techniques. Data Reduction and Statistical Analysis. The Patterson Synthesis. Fourier Techniques. Isomorphous Replacement Phasing. Molecular Replacement. Non-Crystallographic Symmetry. Density Modification. Refinement of Coordinates. Fitting of Maps. Analysis of Coordinates. Protein Crystallography Cookbook. Multiple Isomorphous Replacement. Mutant Studies. Substrate-Analog Example. Molecular Replacement.