Abstract
A general equation relating column performance as a function of important experimental variables is described. Experimental data obtained from studies of effects of particle diameter ( d p ) on efficiency, permeability, and shape of HETP vs. velocity curves for porous silica gel and alumina adsorbents of 5 ⩽ d p ⩽ 40 μm gave a better understanding of the role of new small d p packings in high-performance liquid chromatography. Contrary to the belief that columns packed with porous particles of d p < 10 μm require higher pressures, this study indicates that one may get faster separations with shorter columns at lower pressure with small d p compared to larger d p . Performance of columns packed with porous particles of d p < 20 μm exceeds that of porous layer beads, often used in high-performance liquid—solid chromatography.
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