Abstract
Getting Started. Basics of Separation. Detection Sensitivity and Selectivity. Sample Preparation. The Column. Non--Ionic Samples: Reversed-- and Normal--Phase HPLC. Ionic Samples: Reversed--Phase, Ion--Pair, and Ion--Exchange HPLC. Gradient Elution. Systematic Approach to the Reversed--Phase Separation of Regular Samples. Computer--Assisted Method Development. Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds. Chiral Separations. Preparative HPLC Separation. Quantitation (Including Trace Analysis). Completing the Method: Validation and Transfer. Appendices. Index.
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