Abstract
Applications of liquid Chromatography/nuclear magnetic resonance/mass spectrometry have recently been described which have shown the benefits of this double hyphenation for identification and structure determination. However, the combination of HPLC/NMR and HPLC/MS is not without its difficulties and, as the number of applications of this technology has increased in our laboratory, we have gained considerable insight into the pitfalls that can be encountered when attempting to perform HPLC/NMR/MS. Guidelines are presented to facilitate the effective combination of HPLC with NMR and MS in tandem together with some suggestions as to how the technique may develop. © 1998 John Wiley & Sons, Ltd.
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