Abstract
Liquid-based material and traditional pap smears got during fine-needle biopsies of different tumors were analyzed. Cell-cycle blocks and monolayer pills were made from liquid-based material depending on the amount of cell structures. Original method using donor thrombocyte mass was implemented to create cell-cycle blocks, especially to condense and consolidate the sediment after centrifugation. After taking pancreas punctures different medical institutions in Vladivostok managed to form cell-cycle blocks in 71 cases from 2017 to 2019. In 9 cases (12.7%) the material was found to be diagnostically noninformative, in 15 cases (21.2%) – sufficient for morphological research, but inappropriate for immunohistochemistry. In 47 cases (66.1%) 206 immunohistochemical tests were made using 34 types of biomarkers.
Highlights
For citation: Kotsyrbiy EA, Tychonov YN, Nazarova IV, Reznichek IO, Tumanina AN, Gorelik MZ
Метод клеточных блоков оптимизирует цитологическую диагностику путем увеличения клеточности материала и повышает информативность цитологического исследования за счет возможности проведения иммуногистохимический реакций более чем в 50 % случаев
In 47 cases (66.1 %) 206 immunohistochemical tests were made using 34 types of biomarkers
Summary
For citation: Kotsyrbiy EA, Tychonov YN, Nazarova IV, Reznichek IO, Tumanina AN, Gorelik MZ. MD, PhD, Primorye Regional Pathology Bureau (4 Ostryakova Ave., Vladivostok, 690002, Russian Federation); ORCID: 0000-0001-9186-2914; e-mail: inazarova.cyto@mail.ru При заболеваниях щитовидной железы из-за ее обильного кровоснабжения получение дооперационного материала возможно только при тонкоигольной аспирационной биопсии. Желчных протоков, средостения и других труднодоступных локализаций объем материала, полученного под контролем эндоультразвукового исследования, часто недостаточен для гистологического анализа.
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