Abstract
The limitations of analytical methods and calculations used widely in the estimation of steroid receptor site concentrations and dissociation constants are discussed. Errors arising from loss of steroid during equilibration or analysis or both, the use of excess radioinert steroid to estimate “non-specific” binding, inadequate correction for “non-specific” binding and other miscalculations, loss of receptor during analysis, dilution of radioactive with endogenous steroid and statistical counting errors are considered and assessed by means of theoretical models and of analytical data for progesterone receptors in human endometrium. Four linear plot methods are compared for estimating receptor parameters from experimental data. Single point assay results when compared with estimates of progesterone receptor site concentration by Scatchard analysis do not always give reliable results. On the basis of this study recommendations for further investigations are put forward with a view to improving the quantitative reliability of receptor site determinations.
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