Abstract
Using real-time PCR analysis, the constitutive expression of PR-protein genes encoding thaumatin-like protein (TLP), peroxidase III (TaPero), chitinase (Chitin), glucanase (Glucan), protease inhibitor (PrInh), oxalate oxidase (OxOxid) and lipid transfer protein (Ltp) was studied in collection varieties of winter wheat. It has been shown that plants of varieties with increased resistance to a complex of leaf pathogens have higher constitutive expression levels of Chitin and PrInh genes, and, to a greater extent, of TLP, TaPero and Glucan genes, compared with non-resistant varieties. It is proposed to use constitutive levels of expression of TLP, TaPero and Glucan genes for the selection of winter wheat varietal samples with increased resistance to the complex of leaf diseases.
Highlights
Using real-time PCR analysis, the constitutive expression of PR-protein genes encoding thaumatin-like protein (TLP), peroxidase III (TaPero), chitinase (Chitin), glucanase (Glucan), protease inhibitor (PrInh), oxalate oxidase (OxOxid) and lipid transfer protein (Ltp) was studied in collection varieties of winter wheat
It has been shown that plants of varieties
Предлагается использовать конститутивные уровни экспрессии генов thaumatin-like protein (TLP), TaPero и Glucan в селекционном процессе для отбора сортообразцов озимой пшеницы с повышенной устойчивостью к комплексу листовых болезней
Summary
Using real-time PCR analysis, the constitutive expression of PR-protein genes encoding thaumatin-like protein (TLP), peroxidase III (TaPero), chitinase (Chitin), glucanase (Glucan), protease inhibitor (PrInh), oxalate oxidase (OxOxid) and lipid transfer protein (Ltp) was studied in collection varieties of winter wheat. Цель настоящей работы – сопоставительный анализ конститутивных уровней экспрессии генов PR-белков (TLP, TaPero, Chitin, Glucan, PrInh, OxOxid и Ltp) для выявления генотипов озимой пшеницы с повышенной устойчивостью к комплексу листовых болезней. Объектом исследования были листья зеленых проростков озимой пшеницы (Triticum aestivum L.) коллекционных сортов (Элегия, Августина, Легенда, Короганка, Acratos, Cubus, Skagen, Мера, Лютесценс 1062), предоставленные РУП «НПЦ НАН Беларуси по земледелию».
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More From: Proceedings of the National Academy of Sciences of Belarus, Biological Series
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