Pr and Gd antigens on human B and T lymphocytes and phagocytes

Abstract

Pr 1h, Pr 2, and Pr 3 and Gd antigens were detected on the membrane of human peripheral blood and tonsillary B and T lymphocytes, thymocytes, polymorphonuclear phagocytes, and monocytes using bithermic cytotoxicity and immunofluorescence assays. Generally anti-Pr and anti-Gd had weaker cytotoxic activity, as compared to anti-i and anti-I cold agglutinins. Anti-Pr 1h IgA (R.K.) examined by us had very weak cytotoxicity against peripheral blood lymphocytes but bound to more than 70% of the cells. It reduced E and EAC rosetting capacity of the cells and killed strongly tonsillary B cells, thymocytes, and cord blood polymorphonuclears. Anti-Pr 1h IgM was much more cytotoxic to peripheral blood lymphocytes and to other cells. Anti-Pr 2 had very weak cytotoxic activity and bound to only about half of peripheral blood lymphocytes. Anti-Pr 3 was more cytotoxic to peripheral blood lymphocytes and expressed preferential reactivity against peripheral B and tonsillary B cells. Anti-Gd CA had quite a strong cytotoxic activity against all cells tested.