PPAR－γ激动剂对炎症状态下人系膜细胞LOX－1表达的影响

Abstract

Objective To investigate the effect of peroxisome proliferator-activated receptor γ (PPAR-γ) agonist 15-deoxyprostaglandin J2 (15d-PGJ2) on the expression of lectin-like oxidized low-density lipoprotein (Ox-LDL) receptor 1 (LOX-1) in human glomerular mesangial cells (HMC) treated with interleukin 1β (IL-1β). Methods Laser confocal microscopy was used to observe the effect of IL-1β on lipid uptake by HMC. Real-time quantitative PCR and Western blotting were applied to study the effects of different doses of PPAR-γ agonist 15d-PGJ2 on IL-1β-induced LOX-1 expression. Results Laser confocal microscopy revealed that IL-1β (5 ng/ml) promoted HMC uptake of Ox-LDL. IL-1β promoted the mRNA and protein expression of LOX-1 in HMC, while 15d-PGJ2 dose-dependently inhibited IL-1β-induced LOX-1 expression. Compared with the IL-1β (5ng/ml) group, the treatment group showed that 15d-PGJ2 in concentrations of 2.5 μmol/L, 5 μmol/L, and 10 μmol/L down-regulated the mRNA expression of LOX-1 to 73.13%, 66.54%, and 35.23%, respectively, while 15d-PGJ2 in concentrations of 5 μmol/L and 10 μmol/L down-regulated the protein expression of LOX-1 to 82.39% and 63.17%, respectively. Conclusion Inflammatory factor IL-1β promoted the uptake of Ox-LDL and expression of LOX-1 in HMC. PPAR-γ agonist 15d-PGJ2 dose-dependently inhibited the mRNA and protein expression of LOX-1 induced by IL-1β, which may have a protective role in the lipid imbalance of HMC caused by inflammatory factors. Key words: PPAR-γ agonist; Interleukin-1β; Lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1)