Abstract
Background. Ischemia and reperfusion (I/R) injury is increased with age and may be associated with cardiac peroxisome proliferator-activated receptor (PPAR) activation. The role of specific PPAR isoforms in the heart, particularly with differences in gender and aging, is poorly understood. Purpose. To determine the singular and combined effects of gender and age on recovery from I/R injury and PPAR expression in the rat heart. Methods. Adult (Y) and aged (O) male (M) and female (F) F344 rats were subjected to I/R using Langendorff perfusion (31 min global I; 0.4mM palmitate,1.25mM Ca2+). Recovery function was determined as left ventricular developed pressure and +/−dP/dt. RNA was extracted and real time PCR performed using primer sequences for rat PPARα and PPARβ. Results. A reduction in PPARα and PPARβ mRNA was observed in O compared to Y at baseline. OF had a further reduction of both PPARα and PPARβ mRNA after I/R. Summary. These data suggest, for the first time, that changes in PPARα and PPARβ expression during I/R injury are associated with gender and age.
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