PPARγ, FAS, HSL mRNA and protein expression during Tan sheep fat-tail development

Xiaochun Xu,Wenzhi Niu,Yulin Chen,Xiaolong Wang,Qifang Kou,Yuxin Yang,Xuan Wei

doi:10.1016/j.ejbt.2015.01.004

Abstract

article i nfo Background: The objective of this study was to investigate proliferator-activated receptor (PPARγ), fatty acid synthase (FAS) and hormone-sensitive lipase (HSL) mRNA and protein expression in fat tails of Tan sheep. Rams from different developmental stages (aged 3, 6, 9, 12, 15 and 18 months) were selected, and their tail measurements including length (L), width (W) and girth (G) were recorded. The mRNA and protein expressions of PPARγ, FAS and HSL were examined by quantitative real-time polymerase chain reaction (PCR) and Western blot. Results: The tail measurements increased with age. We observed no significant differences (P N 0.05) of PPARγ mRNA expression between ages 9 and 15 months, and between 12 and 15 months; FAS mRNA expression levels at each developmental stage were observed significantly in Tan sheep (P b 0.05); HSL mRNA expression with no significant differences were only observed between 6 and 15 months (P N 0.05). Significant differences (P b 0.05) of PPARγ, FAS and HSL protein expressions at each developmental stage were observed in Tan sheep. Conclusion: We observed that the mRNA expression patterns of PPARγ and FAS decreased first before they increased again and then this process repeated. Conversely, the mRNA expression patterns of HSL increased first before they decreased and then this process repeated. The protein expression patterns of PPARγ and FAS decreased first before they increased again and then this process repeated. Conversely, the protein expression pattern of HSL increased first before it decreased again and then increased again.

Highlights

This is always a hotspot about fat deposition of livestock for several years [1,2,3,4]
While most studies have concentrated on the fat deposition in subcutaneous [5], intramuscular [6] and visceral adipose tissues [7], relatively little has been done on the tails of sheep
In order to better compare the role of fat deposition in tail development in a Chinese fat-tailed sheep breed (Tan sheep), we examined the mRNA and protein expression patterns of three major candidate genes involved in fat deposition during sheep tail development

Summary

Introduction

This is always a hotspot about fat deposition of livestock for several years [1,2,3,4]. The objective of this study was to investigate proliferator-activated receptor (PPARγ), fatty acid synthase (FAS) and hormone-sensitive lipase (HSL) mRNA and protein expression in fat tails of Tan sheep. Significant differences (P b 0.05) of PPARγ, FAS and HSL protein expressions at each developmental stage were observed in Tan sheep. Conclusion: We observed that the mRNA expression patterns of PPARγ and FAS decreased first before they increased again and this process repeated. The mRNA expression patterns of HSL increased first before they decreased and this process repeated. The protein expression patterns of PPARγ and FAS decreased first before they increased again and this process repeated. The protein expression pattern of HSL increased first before it decreased again and increased again

Objectives

Methods

Results

Conclusion