Abstract
BackgroundThe peroxisome proliferator-activated receptor (PPAR)-γ plays a key role in adipose tissue differentiation and fat metabolism. However, it is unclear which factors may regulate its expression and whether obese patients have changes in adipose tissue expression of PPAR-γor potential regulators such as miR-27. Thus, our aims were to analyze PPAR-γ and miR-27 expression in adipose tissue of obese patients, and to correlate their levels with clinical variables. Subjects and Methods.We included 43 morbidly obese subjects who underwent sleeve gastrectomy (31 of them completed 1-year follow-up) and 19 non-obese subjects. mRNA expression of PPAR-γ1 and PPAR-γ2, miR-27a, and miR-27b was measured by qPCR in visceral and subcutaneous adipose tissue. Clinical variables and serum adipokine and hormone levels were correlated with PPAR-γ and miR-27 expression. In addition, a systematic review of the literature regarding PPAR-γ expression in adipose tissue of obese patients was performed. ResultsWe found no differences in the expression of PPAR-γ and miR-27 in adipose tissue of obese patients vs. controls. The literature review revealed discrepant results regarding PPAR-γ expression in adipose tissue of obese patients. Of note, we described a significant negative correlation between pre-operative PPAR-γ1 expression in adipose tissue of obese patients and post-operative weight loss, potentially linked with insulin resistance markers. ConclusionPPAR-γ1 expression in adipose tissue is associated with weight loss after sleeve gastrectomy and may be used as a biomarker for response to surgery.
Highlights
The pathogenesis of obesity involves the interaction of behavioral, environmental, and genetic factors 1, which results in increased adipose tissue mass due to both adipocyte hypertrophy and hyperplasia, and adipose tissueJorge-Luis Torres and Ricardo Usategui-Martín have contributed to this work as first authors.Luis Muñoz-Bellvís and Miguel Marcos have contributed to this work as senior authors.Extended author information available on the last page of the article dysregulation [2,3]
We included 43 morbidly obese subjects who underwent sleeve gastrectomy (31 of them completed 1-year follow-up) and 19 non-obese subjects. mRNA expression of peroxisome proliferator-activated receptor (PPAR)-γ1 and PPAR-γ2, miR-27a, and miR-27b was measured by quantitative real-time polymerase chain reaction (qPCR) in visceral and subcutaneous adipose tissue
The results showed a negative correlation between PPAR-γ1 expression in adipose tissue and the percentage of weight lost at 12 months and percentage of Body mass index (BMI) reduction at 12 months
Summary
The pathogenesis of obesity involves the interaction of behavioral, environmental, and genetic factors 1, which results in increased adipose tissue mass due to both adipocyte hypertrophy and hyperplasia, and adipose tissueJorge-Luis Torres and Ricardo Usategui-Martín have contributed to this work as first authors.Luis Muñoz-Bellvís and Miguel Marcos have contributed to this work as senior authors.Extended author information available on the last page of the article dysregulation [2,3]. Among the several factors involved in adipose tissue regulation, peroxisome proliferator activated receptor (PPAR)-γ, which is highly expressed in white and brown adipose tissue, is considered the “master regulator” of adipogenesis. This transcription factor is a well-characterized regulator of energy metabolism 6 and is clearly involved in the pathophysiology of obesity [6,7,8] and related complications [9,10]. The peroxisome proliferator-activated receptor (PPAR)-γ plays a key role in adipose tissue differentiation and fat metabolism It is unclear which factors may regulate its expression and whether obese patients have changes in adipose tissue expression of PPAR-γor potential regulators such as miR-27. Conclusion PPAR-γ1 expression in adipose tissue is associated with weight loss after sleeve gastrectomy and may be used as a biomarker for response to surgery
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