PP6 - Assessment of changes in biochemical indices of free radical oxidation in the semen of men living in the Aral Sea region

Abstract

Introduction: At present, the Aral Sea crisis is a global environmental problem, because negative trends in the state of the environment and changes in health outcomes have gained importance for Kazakhstan. In the Aral Sea region a lot of attention is paid to the effects on the body of heavy metals, pesticides, nitrates and other toxicants. The aim of our study was to evaluate changes in biochemical parameters free radical in the semen of men living in the Aral Sea region. Materials and methods: The study served as the sperm of men who live in areas of environmental disaster and environmental crisis, which were divided on three age groups: 1 group - 18 - 29 yrs, 2 group - 30 - 39 yrs, 3 group - 40 - 49 yrs. Determination of malondialdehyde (MDA) was performed by the modified method Korobeynikova Ye.N.(1989). Determination of average molecular method Kovalevskii A.N and Nifanteva O.E. (1990). Results: The study found that in men of all age groups living in the township Shieli zone of ecological crisis level of MDA - in semen decreased relative performance in men living in the city of Aral zone of ecological disaster. To identify toxicity cells was determined content of middle molecules, which are universal biochemical marker of endogenous intoxication in persons living in environmentally disadvantaged areas. Established a significant increase in the level of middle molecules in the semen of men living in the area of environmental crisis, as compared with the men living in the region of ecological disaster. Conclusion: In the zones of ecological crisis and ecological disaster in the men surveyed were found changes in the level of secondary metabolites of lipid peroxidation, malondialdehyde and medium-weight molecules. Spotted an accumulation of catabolites secondary lipid peroxidation MDA and middle molecules in the sperm that indicates a shift of lipid peroxidation and the development of endogenous intoxication in male germ cells, in men living of the Aral Sea region.