PP.42.16

Abstract

Objective: Kinase suppressor of ras 3 (CNKSR3) was a direct mineralocorticoid receptor (MR) target gene that is up-regulated in response to physiological concentrations of aldosterone. Aldosterone is the principal hormonal regulator of sodium homeostasis. Thus, the regulation of CNKSR3 gene is correlated to essential hypertension. The aim of the present study was to assess the association between the human CNKSR3 gene polymorphism and essential hypertension in a Han population of China.Design and method: We use 422 control subjects and 1868 confirmed essential hypertension subjects. All patients and controls were genotyped for the same three single nucleotide polymorphisms SNPs (rs11541340, rs4870285 and rs7757161) of CNKSR3 gene by a real-time PCR instrument. Results: In the Han population, for total, the distribution of rs11541340 and rs7757161 genotypes showed a significant difference between essential hypertension and control subjects (P = 0.044 and p = 0.008). The distribution of rs4870285 genotypes showed no significant difference between essential hypertension and control subjects. The distribution of rs11541340 and rs7757161 recessive model (GT vs GG + TT and CT vs CC + TT, respectively) showed a significant difference between essential hypertension and control participants (P = 0.013 and P = 0.002). The distribution of rs7757161 alleles and dominant model(CC vs CT+TT) showed a significant difference between essential hypertension and control participants (for alleles P = 0.042, P < 0.001). The significant difference of rs11542340 and rs7757161 in recessive model was retained after adjustment for covariates (OR: 2.22, 95% confidence interval [CI]: 1.157–4.259, P = 0.016; OR: 3.125, 95% CI: 1.590–6.143, P = 0.001, respectively). Conclusions: The GG genotype of rs11541340 in CNKSR3 gene could be a protective genetic marker of essential hypertension and C allele may be a protective genetic marker of essential hypertension of Han population in China. The TT genotype of rs7757161 in CNKSR3 gene could be a protective genetic marker of essential hypertension and C allele may be a risk genetic marker of essential hypertension of Han population in China.