PP219 AN INCREASED ARACHIDONIC-TO-EICOSAPENTAENOIC FATTY ACID RATIO IN CELL MEMBRANE ACCELERATES LEAN BODY MASS WASTING FOLLOWING INACTIVITY

Abstract

Rationale: The literature has fewer studies to substantiate lipodystrophy leading to a higher imbalance between free radicals and antioxidants in HIV-infected subjects. The aim of the study was to describe oxidative stress and antioxidant biomarkers status and their association with metabolic and body composition components of HIVlipodystrophy syndrome. Methods: In a cross-sectional study, oxidative stress biomarkers: total hydroperoxide, thiobarbituric acid reactive substances and advanced oxidation protein products (AOPP); antioxidants biomarkers: total glutathione, uric acid and a-tocopherol and metabolic components were determined in blood samples of HIV-infected men with lipodystrophy syndrome (HIV+LIPO = 10); HIVinfected men without lipodystrophy syndrome (HIV = 22) and healthy subjects (Control = 12). Statistical analyzes used ANOVA to compare groups and Spearman correlation coefficient to determine associations between continuous variables in each group. Results: The duration of HIV infection and the duration and type of highly active antiretroviral therapy were similar between two HIV-infected groups. Higher levels of total hydroperoxide were observed in HIV+LIPO (50±33 H2O2/L) compared to both of HIV (19±13 H2O2/L) and Control (5±5 H2O2/L) group (p < 0.05). Higher levels of AOPP were observed in HIV+LIPO (326±173 umol/L) compared to HIV (105±92 umol/L) and Control groups (80±20 umol/L) (p < 0.05). Total hydroperoxide was significantly correlated with insulin serum levels in HIV+LIPO (r = 0.47, p < 0.05) and HIV groups (r = 0.29, p < 0.05). AOPP was significantly correlated with insulin serum levels in HIV+LIPO (r = 0.73, p < 0.05) and HIV (r = 0.54, p < 0.05) groups. Conclusion: Higher lipid and protein oxidation were found in HIV-infected with lipodystrophy syndrome group and both were associated with insulin levels. Disclosure of Interest: None declared