PP-104 Chronic hepatitis B: molecular, epidemiological and clinical features in northwest and central Russia

Abstract

between HBV replication and the expressions of transforming growth factor (TGF)-β1 and insulin-like growth factor-II (IGF-II) in tissues of hepatocellular carcinoma (HCC). Methods: Liver HBV-DNA was detected by in situ molecular hybridization technique, and the expressions of TGF-β1 and IGF-II were detected by the immunohistochemistry, and TGF-β1 mRNA and IGF-II mRNA were amplified by nested-PCR assay in HCCs and their self-control noncancerous tissues. The relationship was investigated between TGF-β1 or IGF-II expression and HBV replication or their clinical pathological characteristics. Results: The stronger expressions (83.3%) of TGF-β1 and IGF-II were found, and the incidences of TGF-β1 mRNA and IGF-II mRNA were 100% in HCC tissues. A significant difference was presented between in HCC tissue and in non-cancerous liver tissues (P 0.05). The levels of TGF-β1 and IGF-II expression were significantly associated with HBV replication with higher HBVDNA-positive HCC (94.7%) than that in HBV-DNA-negative group. Conclusion: TGF-β1 and IGF-II in HCC are overexpressed and associated withhepatic HBV replication and differentiation degree of HCC.