PP014. A pioneer explore of PTZ-induced eclampsia in preeclampsia rats

Abstract

Eclampsia is the most serious state of hypertensive disorder in pregnancy, but the pathogenesis of eclampsia seizures is poorly understood. An eclampsia animal model was developed by PTZ injection on preeclampsia rats. Rats received endotoxin (1.0μg/kg body weight) form tail vein in an hour on GD14 to build the preeclampsia (PE) model. PTZ i.p injection were given to PE rats on GD16, 17, 18 to induce seizures and build an eclampsia model. All experiment rats were divided into three groups, PE, normal, pregnant (P), non-pregnant (NP) group (n=18 in each group). Each group gave two doses of PTZ (40 or 35mg/kg) as subgroups. Blood pressure, urinary albumin, biochemical indexes (AST, ALT, SCr, Urea) were measured. Seizure severity was defined by Racine' standard and seizure time were recorded. PTZ-induced eclampsia shows a increasing blood pressure, urinary albumin, liver function damage and different stages of seizures. The latency of seizure in PE rats with 40mg/kg PTZ is obviously shortened when compared with the P and NP group (p<0.05) and the duration is prolonged (p<0.05). The same trend is still existence at the low dose of PTZ injection. Rate of tonic-clonic seizures is elevated in PE and P group when compared with NP group, but nonsignificantly. We use 40mg/kg PTZ to endotoxin induced preeclampsia model to build an eclampsia model, which mimicked the multiple organ function disorder in human eclampsia. Meanwhile, we found the PE status may decreases threshold for PTZ induced-seizures.