PP.34.40

Abstract

Objective: To characterize the morphology of the brain vessels and glia in experimental modeling of hypertension in rats. Design and method: To create endothelial dysfunction in hypertensive model was reproduced NO deficit in rats in vivo. The animals of the experimental group on a daily basis with the drinking water was administered a NO-synthase blocker as methyl N-nitro-L-arginine (L-NAME, Sigma) for calculating the dose of 50 mg / kg / day. AH development was monitored by measuring the blood pressure of the tail artery at 8 weeks after the administration of L-NAME. (Group 1). The second group consisted of healthy rats that were in standard conditions (group 2). After euthanasia made histological preparations of rat brain, followed by light microscopy - Increase 20. Calculation of results was carried out using Image J. assessed such factors as distribution density of capillaries 1 mm2 (mm/mm), the specific density of the distribution of glial cells. Results: Distribution density of capillaries in the first group was - 32,64 ± 5,1 and 107,01 ± 7,2 in the second. In the first group, a significant decrease in the specific set of cell density neuroglia - 0,62 ± 0,03 as compared with the second group - 4,45 ± 0,05. In the rat model of hypertension microangiopathy showing signs of brain microvascular discharge cells and reduction in the number of neuroglia Conclusions: In the rat model of hypertension microangiopathy showing signs of brain microvascular discharge cells and reduction in the number of neuroglia