Abstract
Aim: The aim of this study was to evaluate the effects of cryotop vitrification of sheep cumulus-oocyte complexes (COCs) on oocyte maturation, apoptotic genes expression and incidence of chromosomal abnormalities. Method: Good quality fresh isolated (Control group) and vitrified warmed COCs (Cryotop group) were matured in vitro for 22 24 hours. The expression of pro(such as Bcl-2) and anti-apoptotic (such as C-myc, P53, Bax & Caspase3) genes was investigated by real-time PCR. Also, the incidence of numerical chromosomal abnormalities was evaluated by cytogenetic analysis. Results: The mean percentage of oocytes in the cryotop and control groups that reached metaphase II was 49.25±3.01% and 51.94±2.7% respectively and there was no significant difference. The expression rates of proand anti-apoptotic genes were similar in both groups, whereas the incidence of numerical chromosomal abnormalities was higher in the cryotop group compared to the control group (42.5% vs. 20%, p < 0.05). Conclusion: Although cryotop vitrification of COCs did not affect the incidence of oocyte maturation or apoptotic genes expression, significant deficiencies in the maintenance of oocyte chromosomal organization were seen.
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