Power Generation and Microbial Community Shift According to Applied Anodic Potential in Electroactive Biofilm Reactors Treating Synthetic and Domestic Wastewater

Abstract

This study investigated the effect of initially set anodic potentials (−0.3, −0.2, −0.1 and +0.1 V) on voltage production and microbial community in electroactive biofilm reactors (EBRs) treating synthetic and domestic wastewater (WW). In phase 1, EBRs were acclimated with different anodic potentials for synthetic and domestic WW. EBR (SE4) poised with +0.1 V showed the highest maximum power density (420 mW/m2) for synthetic WW, while EBR (DE3) poised with −0.1 V showed the highest maximum power density (235 mW/m2) for domestic WW. In phase 2, the EBRs were operated with a fixed external resistance (100 Ω for synthetic WW and 500 Ω for domestic WW) after the applied potentials were stopped. The EBRs showed slightly different voltage productions depending on the WW type and the initial anodic potential, but both EBRs applied with +0.1 V for synthetic (SE4) and domestic (DE4) WW showed the highest voltage production. Principal component analysis results based on denaturing gel gradient electrophoresis band profiles showed that the microbial community was completely different depending on the WW type. Nevertheless, it was found that the microbial community of EBRs applied with a negative potential (−0.3, −0.2, and −0.1 V) seemed to shift to those of EBRs applied with a positive potential (+0.1 V) regardless of WW type. Therefore, positive anodic potential is an important operating factor in electroactive biofilm development and voltage generation for rapid start-up.