POU2F2 promotes the proliferation and motility of lung cancer cells by activating AGO1

Ronggang Luo,Yi Zhuo,Quan Du,Rendong Xiao

doi:10.1186/s12890-021-01476-9

Abstract

BackgroundTo detect and investigate the expression of POU domain class 2 transcription factor 2 (POU2F2) in human lung cancer tissues, its role in lung cancer progression, and the potential mechanisms.MethodsImmunohistochemical (IHC) assays were conducted to assess the expression of POU2F2 in human lung cancer tissues. Immunoblot assays were performed to assess the expression levels of POU2F2 in human lung cancer tissues and cell lines. CCK-8, colony formation, and transwell-migration/invasion assays were conducted to detect the effects of POU2F2 and AGO1 on the proliferaion and motility of A549 and H1299 cells in vitro. CHIP and luciferase assays were performed for the mechanism study. A tumor xenotransplantation model was used to detect the effects of POU2F2 on tumor growth in vivo.ResultsWe found POU2F2 was highly expressed in human lung cancer tissues and cell lines, and associated with the lung cancer patients’ prognosis and clinical features. POU2F2 promoted the proliferation, and motility of lung cancer cells via targeting AGO1 in vitro. Additionally, POU2F2 promoted tumor growth of lung cancer cells via AGO1 in vivo.ConclusionWe found POU2F2 was highly expressed in lung cancer cells and confirmed the involvement of POU2F2 in lung cancer progression, and thought POU2F2 could act as a potential therapeutic target for lung cancer.

Highlights

To detect and investigate the expression of POU domain class 2 transcription factor 2 (POU2F2) in human lung cancer tissues, its role in lung cancer progression, and the potential mechanisms
POU2F2 was highly expressed in human lung cancer tissues and cell lines, and correlated with lung cancer patients’ prognosis To investigate the potential role of POU2F2 in the progression of lung cancer, we first assessed the expression levels of POU2F2 in lung cancer tissues and the adjacent tissues isolated from patients through IHC assays
POU2F2 promotes the proliferation, and motility of lung cancer cells in vitro Since POU2F2 was highly expressed in lung cancer tissues and correlated with the prognosis, we detected its effects on the progression of lung cancer in vitro

Summary

Introduction

To detect and investigate the expression of POU domain class 2 transcription factor 2 (POU2F2) in human lung cancer tissues, its role in lung cancer progression, and the potential mechanisms. In 2018, the mortality of male lung cancer ranked the highest in the world, while lung cancer incidence rate was the highest among women, and the mortality rate was only second [3]. In view of the lack of significant early symptoms of lung cancer, patients are often in an advanced stage [6]. The targeted therapy of lung cancer has made important progress, and a variety of targeted drugs emerge in endlessly [7, 8]. In order to further improve the prognosis of patients, it is still necessary to identify more potential therapeutic targets

Methods

Results

Discussion

Conclusion