Abstract
AbstractThe unicellular freshwater alga Poterioochromonas malhamensis is presented as a screening test system for studying general and specific toxic effects of xenobiotics. The algae are grown in suspension culture in organic substrate and have various characteristics (e.g. fast growth, vegetative reproduction, mobility, etc.) which allow the detection of effects on viability and cell reproduction by light microscopical observation and counting. In addition, the cells exhibit three microtubule‐dependent processes: lorica formation, mitosis and cytokinesis. Using two light microscopical tests adapted to this system (lorica formation test and nuclear phase accumulation test) information about possible interactions of a given test compound with mitotic and/or cytoplasmic microtubules of the algae can be obtained within a comparatively short time (3 hr. and 24 hr. incubation, respectively).
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