Abstract
Potentiometric titrations of iron-sulfur proteins monitored by changes in circular dichroism (CD) or electron-paramagnetic-resonance (EPR) spectrum are presented. Both monitoring techniques have the distinct advantage that interferences caused by the absorption changes of the redox mediators during a titration, which limit the use of the absorption spectrophotometry for monitoring, are either not monitored, or do not represent a serious interference. The CD tritration is illustrated with soluble spinach ferrodoxin and the EPR titration with bound iron-sulfur proteins of green plant photosystem I. The anacrobic titration apparatus and other experimental considerations are described in detail.
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