Abstract
We built an integrated solid-contact ion-selective electrode (SCISE) system with the functionality of self-calibration. A multiplexed SCISE sensor (K+ and NO3− vs. Ag/AgCl) was fabricated on printed-circuit board (PCB) substrates and was subsequently embedded into a microfluidic flow cell for self-calibration and flow-through analysis. A PCB circuit that includes modules for both sensor readout and fluid control was developed. The sensors showed a fast and near-Nernstian response (56.6 for the K+ electrode and −57.4 mV/dec for the NO3− electrode) and maintained their performance for at least three weeks. The sensors also showed a highly reproducible response in an automated two-point calibration, demonstrating the potential for in situ monitoring. Lastly, the sensor system was successfully applied to measure mineral nutrients in plant sap samples.
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