Potentiometric Sensor Based on Molecularly Imprinted Polymer for Determination if Tramadol in A Biologic Fluid Model and Urine

Soroush Sardari

doi:10.34297/ajbsr.2021.12.001743

Abstract

In this study, a potentiometric sensor based on a molecularly imprinted polymer (MIP) was developed for determination of tramadol. At optimized condition the electrode exhibited a Nernestian response (30.5±1.0 mV/decade) in a concentration range of (1×10-6 to 1×10-2 M). The potential response of the electrode was constant in the pH range of 1.0-9.0. The electrode demonstrated a response time about 15s. Selectivity coefficient of the sensor with structural similarity to tramadol was evaluated by matched potential method (MPM). The electrode was examined successfully for determination of tramadol in biological fluids and urine with a good accuracy and precision

Highlights

Over the last few years, molecular imprinting technique has been received high attention in the field of polymer science and chemical analysis, owing to its predetermined selectivity for target molecular, high affinity and robustness [1,2]
The influence of membrane composition on the potential response was tested and the results shown in (Table 1)
The pH dependence of the membrane sensor was tested with tramadol concentration of 10-4 M in the pH 1-14 that the results shown in (Figure 3)

Summary

Introduction

Over the last few years, molecular imprinting technique has been received high attention in the field of polymer science and chemical analysis, owing to its predetermined selectivity for target molecular, high affinity and robustness [1,2]. After removing of the template molecules from the polymerized material, binding sites of the MIP with molecular recognition properties are formed. Potentiometric sensors are generic and highly successful They do not require the template molecules to diffuse through the electrode membranes for generation of membrane potentials have shown to be very promising, unlike sensors based other transduction techniques, so the MIP based potentiometric sensors are very promising and have gotten a rapid response time but still the reports of MIP based potentiometric sensor are rather rare [14,15,16,17,18,19]

Methods

Results

Conclusion