Potentilla rugulosa Nakai Extract Attenuates Bisphenol A-, S- and F-Induced ROS Production and Differentiation of 3T3-L1 Preadipocytes in the Absence of Dexamethasone.

Sun-Il Choi,Jong Seok Lee,Sarah Lee,Ok-Hwan Lee,Wan-Sup Sim,Young-Cheul Kim

doi:10.3390/antiox9020113

Abstract

Endocrine disrupting chemicals (EDCs) disrupt the physiological metabolism, thus playing an important role in the development of obesity. EDCs, the so-called ‘obesogens’, might predispose some individuals to gain weight. This study investigated the effects of bisphenol A (BPA) and its alternatives (BPS and BPF) on adipocyte differentiation and the effects of the leaves of Potentilla rugulosa Nakai extract (LPE) as a functional food ingredient on obesogen-induced lipid production and adipogenesis in 3T3-L1 cells. The results showed that LPE has high total phenolic and flavonoid contents (77.58 ± 0.57 mg gallic acid equivalents (GAE)/g and 57.31 ± 1.72 mg quercetin equivalents (QE)/g, respectively). In addition, LPE exerted significant antioxidant effects in terms of DPPH radical scavenging activity, reducing power, ferric-ion reducing antioxidant power, and oxygen radical absorbance capacity. BPA, BPS, and BPF increased lipid accumulation, protein expressions of adipogenic transcription factors (PPAR-γ, C/EBP-α, and aP2), and reactive oxygen species (ROS) production in 3T3-L1 cells. However, LPE suppressed the BPA-, BPS-, and BPF-induced effects on adipogenesis. Therefore, LPE has potential as a functional food supplement that can prevent bisphenol-induced lipid metabolism disorders.

Highlights

The increasing incidence of obesity is a serious global public health concern
57.31 ± 1.72 mg QE/g of total phenolic compounds. These results are in good agreement with those of another study by Tomczyk et al [19], who determined total phenolic content of seven other species of the Potentilla genus
We investigated whether leaves of Potentilla rugulosa Nakai extract (LPE) could regulate the bisphenol A (BPA), bisphenol S (BPS), and bisphenol F (BPF)-induced differentiation of 3T3-L1 preadipocytes into mature adipocytes

Summary

Introduction

The causes of obesity are over-ingestion, sedentary lifestyle, changing eating habits, and genetic factors. These factors alone do not completely support the current rise in obesity. The production and use of synthetic resins increased rapidly in parallel with the increase in diabetes epidemic during the same period [2]. This correlation, coupled with experimental evidence demonstrating that certain environmental pollutants induce adipogenesis and weight gain in experimental models, led to the environmental obesogen hypothesis that posits a causative role of synthetic chemicals in the pathogenesis of obesity [3]. Obesogens can be Antioxidants 2020, 9, 113; doi:10.3390/antiox9020113 www.mdpi.com/journal/antioxidants

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