Potentials of neuron-specific enolase as a biomarker for gastric cancer

Lingyun Wang,Ruirui Hu,Hongmei Liu,Lili Zhou,Yuanyuan Ding,Xiangyu Liu,Wen Li

doi:10.4314/tjpr.v19i3.7

Abstract

Purpose: To investigate the potentials of neuron-specific enolase (NSE) as a biomarker for gastric cancer (GC). Methods: Gastric cancer (GC) patients (n = 412) who underwent gastrectomy were recruited over a 3- year period for this study. Their clinicopathological data such as age, sex, histological type, depth, tumor invasion, lymph node metastasis, and distant metastasis were analyzed. The patients were followed up for four years and the outcomes were also assessed. Histological changes in biopsies and levels of expression of NSE in biopsies and serum of patients were determined using immunohistochemical staining, western blotting and enzyme-linked immunosorbent assay (ELISA), respectively. Results: Immunohistochemical staining showed that NSE was differentially expressed in the cytoplasm of GC cells. Histological changes in biopsies of patients in the overexpression group were not significantly different from those of patients in under-expression group (p > 0.05). In NSE overexpression group, the number of patients in early stage GC subgroup (n = 186, 86.10 %, T1) were significantly higher than that in advanced GC subgroup (n = 124, 62.20 % T2–T4) (p < 0.05). However, in NSE under-expression group, there were more patients in advanced GC subgroup (n = 72, 37.70 %) than in early GC subgroup (n = 30, 13.80 %) (p < 0.05). Patients in NSE overexpression group survived longer than those in NSE under-expression group (p < 0.05). The level of expression of NSE significantly decreased with increase in TNM stage (p < 0.05). There was no significant difference in serum NSE level between GC patients and healthy control (p > 0.05). The results of the correlation analysis indicated that NSE levels were positively associated with GC. Conclusion: The results obtained in this study suggest that NSE could serve as a potential biomarker for GC. Keywords: Biomarker, Gastric cancer, Neuron-specific enolase, Overexpression, TNM staging

Highlights

Gastric cancer (GC) is a common digestive tract cancer characterized by high morbidity and mortality
Patients with GC overexpressing neuron-specific enolase (NSE) usually have better prognosis, suggesting that NSE could serve as a potential biomarker for GC [7]
Subtotal resection was performed on 298 patients, total resection involved 84 patients, while proximal gastrectomy was done on 30 patients

Summary

Introduction

Gastric cancer (GC) is a common digestive tract cancer characterized by high morbidity and mortality. It can be treated if diagnosed early, the 5-year survival and overall prognosis are unsatisfactory. It is the fifth most common type of cancer in males, and the fourth most. Human enolase comprises three subunits: alpha (α), beta (β), and gamma (γ), each encoded by a separate gene. These forms can combine to form five different isoenzymes: αα, αβ, αγ, ββ, and γγ. The enzyme is used as a marker for SCLC [5,6]

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Conclusion