Potential Uses of Xylanase-Rich Lignocellulolytic Enzymes Cocktail for Oil Palm Trunk (OPT) Degradation and Lignocellulosic Ethanol Production

Abstract

In this paper, we reported that oil pam trunk (OPT) can be used as an alternative fermentation feedstock for lignocellulolytic enzyme production and carbon source for bioethanol production. Xylanase production from OPT by locally isolated fungus, Aspergillus fumigatus SK1, under solid-state fermentation (SSF), was optimized using central composite design (CCD). Under optimized conditions, a maximum xylanase activity of 1792.43 U/g was produced, which was 4.28-fold higher than before optimization. Significant amounts of CMCase (56.19 U/g), FPase (3.47 U/g), and β-glucosidase (1.55 U/g) were also found concomitantly with xylanase. Subsequently, the effect of solid loading, Tween-80 concentration, and incubation temperature on the saccharification of OPT by the crude enzymes were optimized to enhance the total reducing sugar production. A total of 13.148 g/L of reducing sugar was reported under optimized conditions. Comparisons of physiochemical characteristics between native and hydrolyzed OPT via scanning ...