Potential use of grapevine cv Askari for heavy metal phytoremediation purposes at greenhouse scale.

Abstract

Grapevine varieties possess desirable characteristics for phytoremediation purposes. We investigated the potential of grapevine cv Askari in phytoremediation of heavy metal (HM) pollutions. In total 80 grapevine seedlings were exposed to four levels of HM stress (mild, low, moderate, and severe) in greenhouse condition during two growing years (2018 and 2019). The HM concentrations (Zn, Cu, Cd, Cr, and Pb) were subsequently determined in the soils, roots, and grapevine aboveground parts (AGPs), and then phytoextraction and phytostabilization potential assessment indices, i.e., biological absorption coefficient (BAC), bioconcentration factor (BCF), and translocation factor (TF), were calculated. Results led to ranking of the cumulative concentration order of the HMs, i.e.: soils (3476.6) > AGPs (1418.8) > roots (562.2) mg/kg-DW. The mean concentrations ranking order of studied HMs were in soil: Cu (1184.8) > Pb (865.5) > Cd (803.2) > Cr (623.0) > Zn (277.9) mg/kg-DW; roots, Cu (242.0) > Cd (239.5) > Zn (188.8) > Pb (63.5) > Cr (17.2) mg/kg-DW; and AGP environments, Cu (910.2) > Cd (322.9) > Zn (160.3) > Pb (152.9) > Cr (25.3) mg/kg-DW. Principal component analysis results demonstrated the same distribution pattern for the studied HMs between soil, root, and AGP environments, and the highest correlation coefficients were found for Cu, Zn, and Cd. Based on the obtained results (Cu-BAC (> 1), Zn-BCF (> 1), Zn-TF (< 1), Cu-AGP mean concentrations (> 1000 mg/kg-DW), and Cd-AGP mean concentrations (> 100 mg/kg-DW)), it can be concluded that grapevine cv Askari possesses potentials for phytoremediation purposes of Cu, Zn, and Cd. These results were acquired in a greenhouse environment and under controlled conditions; we suggest that the phytoremediation potential of this grapevine variety be assessed in a contaminated vineyard environment as well.