Abstract
Extracellular polymeric substances (EPS) can be used as an effective biosorbent for removing heavy metals from soil washing effluent. In this research, Bacillus subtilis was used as an EPS-producing bacteria with a yield of bound EPS of 0.6 mg/g biomass with protein and polysaccharide content of 199 mg/g EPS and 8.8 mg/g EPS, respectively. The maximum biosorption capacities for mercury in artificial wastewater using bound EPS and dead biomass of Bacillus subtilis as biosorbents were 9.32 mg/g and 6.04 mg/g, respectively, at a biosorbent dose of 0.1 g/100 mL. Biosorption using Bacillus subtilis fitted the Langmuir isotherm, while biosorption using bound EPS fitted the Freundlich isotherm. The biosorption process followed a pseudo-second-order kinetic model for both biosorbents. Bound EPS was also feasible to treat soil washing effluent with a biosorption capacity of 2.21 mg/g of mercury. The use of bound EPS could effectively remove mercury from wastewater.
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