Abstract
Background : Prenatal paternity test is mostly performed by using Amniocentesis or Chorionic Villus Sampling (CVS) methods. However, these methods require invasive procedures, which are potentially harmful for both the mother and the fetus. Currently, the invention of of Cell-Free Fetal DNA (cffDNA) has offered the opportunity of performing prenatal paternity test non-invasively. Materials and Methods : This study is a cross sectional descriptive study to detect cell free fetal DNA at 13 STR loci and at amelogenin gene to evaluate fetus gender, which will be compared to the baby gender afterbirth. Healthy third semester pregnant women were included as participants. Inform consent for both the mother and the biological father has been provided. Result : Four participants has been evaluated. In this study, in all participants, we found the presence of cffDNA in almost all of the STR loci. Some loci cannot be detected due to the small amount of cffDNA in the loci. All fetus genders detected by cffDNA in the amelogenin gene macthed the gender of the four babies afterbirth. Conclusion : The use of Cell-Free Fetal DNA (cffDNA) is a potential non-invasive methods in prenatal paternity test. Additionally, the ability of the method to evaluate fetus gender has been suggested.
Highlights
During pregnancy, the fetal and maternal circulation is separated by the placental membrane
In all participants, we found the presence of Cell-Free Fetal DNA (cffDNA) in almost all of the short tandem repeat (STR) loci
This study aims to detect the presence of cell free fetal DNA at 13
Summary
The fetal and maternal circulation is separated by the placental membrane. Various evidences have pointed to the incompleteness of these barriers to cellular exchange.[1] Several hypotheses regarding the origin of fetal DNA in the maternal circulation are fetal hemapoetic cells, placental trophoblast and transferred directly from the maternal. One possible explanation is that fetal DNA comes from fetal cells that were damaged when trying to enter the maternal circulation. Another hypothesis states that a high enough fetal DNA concentration is thought to originate from apoptotic placental trophoblasts, so that the concentration of fetal. These methods require invasive procedures, which are potentially harmful for both the mother and the fetus. Has offered the opportunity of performing prenatal paternity test non-invasively
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