Potential therapeutic roles of tanshinone IIA in human bladder cancer cells.

Sheng-Chun Chiu,Chi-Cheng Chen,Tsung-Hsun Tsai,Shee-Ping Chen,Cheng-Yoong Pang,Sung-Ying Huang,Shang-Sen Lee,Teng-Fu Hsieh,Tien-Huang Lin,Shu-Fang Chang,Hsin-Ho Liu

doi:10.3390/ijms150915622

Abstract

Tanshinone IIA (Tan-IIA), one of the major lipophilic components isolated from the root of Salviae Miltiorrhizae, has been found to exhibit anticancer activity in various cancer cells. We have demonstrated that Tan-IIA induces apoptosis in several human cancer cells through caspase- and mitochondria-dependent pathways. Here we explored the anticancer effect of Tan-IIA in human bladder cancer cell lines. Our results showed that Tan-IIA caused bladder cancer cell death in a time- and dose-dependent manner. Tan-IIA induced apoptosis through the mitochondria-dependent pathway in these bladder cancer cells. Tan-IIA also suppressed the migration of bladder cancer cells as revealed by the wound healing and transwell assays. Finally, combination therapy of Tan-IIA with a lower dose of cisplatin successfully killed bladder cancer cells, suggesting that Tan-IIA can serve as a potential anti-cancer agent in bladder cancer.

Highlights

IntroductionCalmette-Guérin (BCG) immunotherapy with 5-year survival rate approaches 90%, but for invasive
Bladder cancer (BCa) ranks sixth in cancer incidence in the United States [1]
By using various bladder cancer cell lines, we delineated the mechanism of how Tanshinone IIA (Tan-IIA) killed these bladder cancer cells

Summary

Introduction

Calmette-Guérin (BCG) immunotherapy with 5-year survival rate approaches 90%, but for invasive. 10% 5-year survival rate for the metastatic BCa [3]. The high recurrence rate of BCa has raised the cost of treatment from $96,000 to $187,000 per BCa patient (from diagnosis to death) [4], and with an estimated total cost of $3.98 billion in 2010 [5]. The antitumor activity of Tan-IIA is mainly due to proliferation inhibition and apoptosis induction [11,12,13]. Tan-IIA decreases human cancer cells invasion and metastasis [16,17,18,19]. No investigation on Tan-IIA and bladder cancer has been disclosed to date. We conducted a series of experiments to investigate the effect of

Results

Discussion

Conclusion