Potential sulfate reducing bacteria for decrease Fe and Mn determination agents on ex-coal mining soil in Samarinda

Abstract

The aim of this study was to determine the potential of sulfate reducing bacteria (SRB) as reducing agents for decrease Fe and Mn in acid soils of coal mines using five isolates of isolated SRB from post-coal mining sediments in Samarinda. The SRB potential test in reducing Fe and Mn was carried out by growing SRB on liquid Postgate media with varying pH of 3, 4 and 5 with the addition of mine acid soil to each treatment. Each of the pure SRB isolates (1 ml) was inoculated into liquid Postgate media with different pH, namely pH 3, 4 and 5 which had previously been filled with 5 grams of acidic soil after coal mining which had been mashed and sterilized at 121°C for 15 minutes. The culture was incubated in a threaded tube of 10 ml volume to full. The results showed that five isolates of SRB from post-coal mine sediments in Samarinda were Desulfococcus sp., Desulfotomaculum sp., Desulfobacter sp., Desulfobulbus sp. and Desulfobacterium sp. have potential as reducing agents for decrease Fe and Mn in Postgate B liquid media which have been added with coal mine acid soils. The best efficiency of Fe reduction by Desulfobacterium sp. at pH 3 is 6% and the best Mn reduction by bacteria Desulfotomaculum sp. at pH 5 is 4%.