Potential semen quality rate of Ongole Crossbred Bulls at Cattle Breeding Station (UPT PT & HMT) Tuban - Indonesia

Abstract

The variation in semen quality serves to determine the fertility potential of Ongole Crossbred (PO) bulls. Semen quality has a correlation to fertility. Individual motility is one of most important factors determining the success of artificial insemination program. The aims of this research were to mapping the bulls who have high and low fertility potential. This variation is used to determine the composition of molecular marker genes for reproductive traits through the RNA-Sequencing method. 12 PO bulls aged 2–8 years were used in this research. The research parameters were volume, concentration, individual motility, viability, abnormality and sexual behaviour. Sexual behaviour data were analyzed using descriptive methods. Semen quality data were analyzed using an unpaired T-test. The results showed that the bulls code with the highest to lowest motility values were PO-5, PO-2, PO-3, PO-6, PO-4, PO-7, PO-9, PO-8, PO-1, PO-10, PO-11, PO-12, respectively. 4 bulls with the highest motility values and the 4 bulls with the lowest motility values had significant differences (P<0.05). Therefore, it can be concluded that the two populations of PO bulls with the highest and lowest motility value are different. It can be continued with the RNA-Sequencing method to find the gene composition of the spermatozoa.